Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CRTC1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPSC derived neural cells
NA
NA

Attributes by original data submitter

Sample

source_name
CRTC1 ChIP-Seq, iCell neurons, unstimulated, biorep 4, tech rep 1
tissue
cultured human iPSC-derived neurons at ~DIV16
time post-kcl stimulation
unstimulated
biological replicate
4
technical replicate
1
chip epitope
CRTC1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
nuclear isolation from homogenized cultures Nugen Ovation Ultralow Library System V1 using 2ng of starting DNA and performing 18 cycles of amplification

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
55257883
Reads aligned (%)
90.1
Duplicates removed (%)
36.1
Number of peaks
1401 (qval < 1E-05)

hg19

Number of total reads
55257883
Reads aligned (%)
89.5
Duplicates removed (%)
37.8
Number of peaks
1074 (qval < 1E-05)

Base call quality data from DBCLS SRA